Multimodal optical mesoscopy reveals the quantity and spatial distribution of gram-positive biofilms in ex vivo tonsils
Clapperton, Megan and Kunanandam, Tash and Florea, Catalina D. and Douglas, Catriona M. and McConnell, Gail (2023) Multimodal optical mesoscopy reveals the quantity and spatial distribution of gram-positive biofilms in ex vivo tonsils. Other. bioRxiv, Ithaca, NY. (https://doi.org/10.1101/2023.07.03.547470)
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Abstract
Biofilms are known to be present in tonsils, but little is known about their spatial location and size distribution throughout the tonsil. Studies of the location and distribution of biofilms in tonsil specimens have thus far been limited to either high-magnification methods such as electron microscopy, which enables high resolution imaging but only from a tiny tissue volume, or lower magnification techniques such as light microscopy, which allow imaging of larger specimens but with poor spatial resolution. To overcome these limitations, we report the use of multimodal optical mesoscopy to visualize and quantify the number and spatial distribution of gram-positive biofilms in fresh, excised paediatric tonsils. This methodology supports simultaneous imaging of both the tonsil host and biofilms in whole mounts of tissue up to 5 mm x 5 mm x 3 mm with subcellular resolution throughout. A quantitative assessment of thirty-six tonsil specimens revealed no statistically significant difference between biofilm presence on the tonsil surface and the interior of the tonsil. This new quantitative mesoscale imaging approach may prove useful in understanding the role of biofilms in tonsillar diseases and other infections.
ORCID iDs
Clapperton, Megan, Kunanandam, Tash, Florea, Catalina D., Douglas, Catriona M. and McConnell, Gail ORCID: https://orcid.org/0000-0002-7213-0686;-
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Item type: Monograph(Other) ID code: 86452 Dates: DateEvent3 July 2023PublishedSubjects: Science > Physics > Optics. Light
Science > MicrobiologyDepartment: Faculty of Science > Physics
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical SciencesDepositing user: Pure Administrator Date deposited: 11 Aug 2023 15:28 Last modified: 08 Oct 2024 00:06 URI: https://strathprints.strath.ac.uk/id/eprint/86452