The development of a novel pipeline for polymeric nanoparticle characterization following incubation under shear flow conditions

Daramy, Karim and Punnabhum, Panida and Perrie, Yvonne and Rattray, Zahra (2023) The development of a novel pipeline for polymeric nanoparticle characterization following incubation under shear flow conditions. In: National Physical Laboratory PGI conference: Postgraduate Institute for Measurement Science Symposium 2023, 2023-06-06 - 2023-06-08, University of Strathclyde. (Unpublished)

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Abstract

Nanoparticles are small colloidal particles with a dimension between 1-100 nm in size. Polymeric nanoparticles are routinely explored for the development of novel medicines for unmet clinical need due to their unique properties. However, there is currently a high attrition rate for bench-clinic translation, and this may be due to a lack of understanding of the behaviour of nanoparticles under physiologically-relevant conditions. Upon administration to protein-containing medium, nanoparticles will spontaneously adsorb proteins onto their surface and form what is known as the ‘protein corona’ (figure 1) . Protein corona formation leads to changes in the physical and chemical parameters of nanoparticles, which subsequently alters their biological fate (cellular uptake, biodistribution). With most nanoparticles intended for intravenous administration, it is therefore crucial to characterize the impact of biological shear flow conditions on nanoparticle-protein interactions and how this impact their colloidal stability. Aims & Objectives: 1)To develop a robust pipeline for the reproducible characterization of nanoparticles following protein corona formation under physiologically relevant shear flow conditions. 2) To use a range of nanoparticle isolation and analytical techniques to measure the impact of sample handling conditions on particle parameters using model nanoparticles (polystyrene latex).

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