4D tracking of clinical seminal samples for quantitative characterization of motility parameters

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Di Caprio, Giuseppe and El Mallahi, Ahmed and Ferraro, Pietro and Dale, Roberta and Coppola, Gianfranco and Dale, Brian and Coppola, Giuseppe and Dubois, Frank (2014) 4D tracking of clinical seminal samples for quantitative characterization of motility parameters. Biomedical Optics Express, 5 (3). pp. 690-700. ISSN 2156-7085 (https://doi.org/10.1364/BOE.5.000690)

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Abstract

In this paper we investigate the use of a digital holographic microscope, with partial spatial coherent illumination, for the automated detection and tracking of spermatozoa. This in vitro technique for the analysis of quantitative parameters is useful for assessment of semen quality. In fact, thanks to the capabilities of digital holography, the developed algorithm allows us to resolve in-focus amplitude and phase maps of the cells under study, independently of focal plane of the sample image. We have characterized cell motility on clinical samples of seminal fluid. In particular, anomalous sperm cells were characterized and the quantitative motility parameters were compared to those of normal sperm.

ORCID iDs

Di Caprio, Giuseppe ORCID logoORCID: https://orcid.org/0000-0001-5564-8064, El Mallahi, Ahmed, Ferraro, Pietro, Dale, Roberta, Coppola, Gianfranco, Dale, Brian, Coppola, Giuseppe and Dubois, Frank;
CORE (COnnecting REpositories)