Label-free imaging and biochemical characterization of bovine sperm cells

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Ferrara, Maria Antonietta and Di Caprio, Giuseppe and Managò, Stefano and De Angelis, Annalisa and Sirleto, Luigi and Coppola, Giuseppe and De Luca, Anna Chiara (2015) Label-free imaging and biochemical characterization of bovine sperm cells. Biosensors, 5 (2). pp. 141-157. ISSN 2079-6374 (https://doi.org/10.3390/bios5020141)

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Abstract

A full label-free morphological and biochemical characterization is desirable to select spermatozoa during preparation for artificial insemination. In order to study these fundamental parameters, we take advantage of two attractive techniques: digital holography (DH) and Raman spectroscopy (RS). DH presents new opportunities for studying morphological aspect of cells and tissues non-invasively, quantitatively and without the need for staining or tagging, while RS is a very specific technique allowing the biochemical analysis of cellular components with a spatial resolution in the sub-micrometer range. In this paper, morphological and biochemical bovine sperm cell alterations were studied using these techniques. In addition, a complementary DH and RS study was performed to identify X- and Y-chromosome-bearing sperm cells. We demonstrate that the two techniques together are a powerful and highly efficient tool elucidating some important criterions for sperm morphological selection and sex-identification, overcoming many of the limitations associated with existing protocols.

ORCID iDs

Ferrara, Maria Antonietta ORCID logoORCID: https://orcid.org/0000-0001-6216-2572, Di Caprio, Giuseppe ORCID logoORCID: https://orcid.org/0000-0001-5564-8064, Managò, Stefano, De Angelis, Annalisa, Sirleto, Luigi, Coppola, Giuseppe and De Luca, Anna Chiara;
CORE (COnnecting REpositories)