Laboratory evaluation of the broad-spectrum antibacterial efficacy of a low-irradiance visible 405-nm light system for surface-simulated decontamination

Sinclair, Lucy G and Dougall, Laura R and Ilieva, Zornitsa and McKenzie, Karen and Anderson, John G and MacGregor, Scott J and Maclean, Michelle (2023) Laboratory evaluation of the broad-spectrum antibacterial efficacy of a low-irradiance visible 405-nm light system for surface-simulated decontamination. Health and Technology, 13 (4). pp. 615-629. ISSN 2190-7196 (https://doi.org/10.1007/s12553-023-00761-3)

[thumbnail of Sinclair-etal-HT-2023-Laboratory-evaluation-of-the-broad-spectrum-antibacterial-efficacy-of-a-low-irradiance-visible-405-nm-light-system]
Preview
 Text. Filename: Sinclair_etal_HT_2023_Laboratory_evaluation_of_the_broad_spectrum_antibacterial_efficacy_of_a_low_irradiance_visible_405_nm_light_system.pdf
Final Published Version
License: Creative Commons Attribution 4.0 logo
Download (2MB)| Preview

Abstract

Purpose: Lighting systems which use visible light blended with antimicrobial 405-nm violet-blue light have recently been developed for safe continuous decontamination of occupied healthcare environments. This paper characterises the optical output and antibacterial efficacy of a low irradiance 405-nm light system designed for environmental decontamination applications, under controlled laboratory conditions. Methods: In the current study, the irradiance output of a ceiling-mounted 405-nm light source was profiled within a 3×3×2 m (18 m 3) test area; with values ranging from 0.001-2.016 mWcm -2. To evaluate antibacterial efficacy of the light source for environmental surface decontamination, irradiance levels within this range (0.021-1 mWcm -2) at various angular (Δ ϴ=0-51.3) and linear (∆s=1.6-2.56 m) displacements from the source were used to generate inactivation kinetics, using the model organism, Staphylococcus aureus. Additionally, twelve bacterial species were surface-seeded and light-exposed at a fixed displacement below the source (1.5 m; 0.5 mWcm -2) to demonstrate broad-spectrum efficacy at heights typical of high touch surfaces within occupied settings. Results: Results demonstrate that significant (P≤0.05) inactivation was successfully achieved at all irradiance values investigated, with spatial positioning from the source affecting inactivation, with greater times required for inactivation as irradiance decreased. Complete/near-complete (≥93.28%) inactivation of all bacteria was achieved following exposure to 0.5 mWcm -2 within exposure times realistic of those utilised practically for whole-room decontamination (2-16 h). Conclusion: This study provides fundamental evidence of the efficacy, and energy efficiency, of low irradiance 405-nm light for bacterial inactivation within a controlled laboratory setting, further justifying its benefits for practical infection control applications.

ORCID iDs

Sinclair, Lucy G ORCID logoORCID: https://orcid.org/0009-0007-8121-7795, Dougall, Laura R ORCID logoORCID: https://orcid.org/0000-0002-3514-1853, Ilieva, Zornitsa, McKenzie, Karen, Anderson, John G ORCID logoORCID: https://orcid.org/0000-0003-4151-1619, MacGregor, Scott J ORCID logoORCID: https://orcid.org/0000-0002-0808-585X and Maclean, Michelle ORCID logoORCID: https://orcid.org/0000-0001-5750-0397;
CORE (COnnecting REpositories)