Signal amplification in electrochemical DNA biosensors using target-capturing DNA origami tiles

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Williamson, Paul and Piskunen, Petteri and Ijäs, Heini and Butterworth, Adrian and Linko, Veikko and Corrigan, Damion K. (2023) Signal amplification in electrochemical DNA biosensors using target-capturing DNA origami tiles. ACS Sensors, 8 (4). pp. 1471-1480. ISSN 2379-3694 (https://doi.org/10.1021/acssensors.2c02469)

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Abstract

Electrochemical DNA (e-DNA) biosensors are feasible tools for disease monitoring, with their ability to translate hybridization events between a desired nucleic acid target and a functionalized transducer, into recordable electrical signals. Such an approach provides a powerful method of sample analysis, with a strong potential to generate a rapid time to result in response to low analyte concentrations. Here we report a strategy for the amplification of electrochemical signals associated with DNA hybridization, by harnessing the programmability of the DNA origami method to construct a sandwich assay to boost charge transfer resistance (RCT) associated with target detection. This allowed for an improvement in sensor limit of detection by two-orders of magnitude compared to a conventional label-free e-DNA biosensor design and linearity for target concentrations between 10 pM – 1 nM without the requirement for probe labeling, or enzymatic support. Additionally, this sensor design proved capable of achieving a high degree of strand selectivity in a challenging DNA-rich environment. This approach serves as a practical method for addressing strict sensitivity requirements necessary for a low cost point-of-care (PoC) device.

ORCID iDs

Williamson, Paul, Piskunen, Petteri, Ijäs, Heini, Butterworth, Adrian ORCID logoORCID: https://orcid.org/0000-0002-0463-927X, Linko, Veikko and Corrigan, Damion K. ORCID logoORCID: https://orcid.org/0000-0002-4647-7483;
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