Investigating centrifugal filtration of serum-based FTIR spectroscopy for the stratification of brain tumours

Theakstone, Ashton G. and Brennan, Paul M. and Jenkinson, Michael D. and Goodacre, Royston and Baker, Matthew J. (2023) Investigating centrifugal filtration of serum-based FTIR spectroscopy for the stratification of brain tumours. PLoS One, 18 (2). e0279669. ISSN 1932-6203 (https://doi.org/10.1371/journal.pone.0279669)

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Abstract

Discrimination of brain cancer versus non-cancer patients using serum-based attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy diagnostics was first developed by Hands et al with a reported sensitivity of 92.8% and specificity of 91.5%. Cameron et al. then went on to stratifying between specific brain tumour types: glioblastoma multiforme (GBM) vs. primary cerebral lymphoma with a sensitivity of 90.1% and specificity of 86.3%. Expanding on these studies, 30 GBM, 30 lymphoma and 30 non-cancer patients were selected to investigate the influence on test performance by focusing on specific molecular weight regions of the patient serum. Membrane filters with molecular weight cut offs of 100 kDa, 50 kDa, 30 kDa, 10 kDa and 3 kDa were purchased in order to remove the most abundant high molecular weight components. Three groups were classified using both partial least squares-discriminate analysis (PLS-DA) and random forest (RF) machine learning algorithms; GBM versus non-cancer, lymphoma versus non-cancer and GBM versus lymphoma. For all groups, once the serum was filtered the sensitivity, specificity and overall balanced accuracies decreased. This illustrates that the high molecular weight components are required for discrimination between cancer and non-cancer as well as between tumour types. From a clinical application point of view, this is preferable as less sample preparation is required.

  • Item type:Article
    ID code:84294
    Dates:
    Date
    Event
    17 February 2023
    Published
    15 February 2023
    Accepted
    12 December 2022
    Submitted
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:20 Feb 2023 10:47
    Last modified:27 Apr 2024 13:34
    URI:https://strathprints.strath.ac.uk/id/eprint/84294
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