Rapid assessment of antibiotic susceptibility using a fully 3D-printed impedance- based biosensor
Domingo-Roca, R. and Lasserre, P. and Riordan, L. and Macdonald, A.R. and Dobrea, A. and Duncan, K.R. and Hannah, S. and Murphy, M. and Hoskisson, P.A. and Corrigan, D.K. (2023) Rapid assessment of antibiotic susceptibility using a fully 3D-printed impedance- based biosensor. Biosensors and Bioelectronics X, 13. 100308. ISSN 2590-1370 (https://doi.org/10.1016/j.biosx.2023.100308)
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Abstract
The sustained misuse and overuse of antibacterial agents is accelerating the emergence of antimicrobial resistance (AMR), which is becoming one of the major threats to public health. Abuse of antibiotics drives spontaneous evolution, bacterial mutation, and exchange of resistant genes through lateral gene transfer. Mitigating the worldwide impact of AMR requires enhanced antibiotic stewardship through faster diagnostic testing. In this work, we aim to tackle this issue via development of a fully 3D-printed electrochemical, gel-modified biosensor for rapid bacterial growth monitoring. By using electrochemical impedance spectroscopy, we have successfully identified growth profiles and confirmed antibiotic susceptibility of two ESKAPE pathogens, Escherichia coli and Pseudomonas aeruginosa, following overnight culture it was possible to determine antibiotic sensitivity in 90 min, altogether faster than the 24–48 h current gold standard of culture-based antimicrobial susceptibility testing with significant scope for optimisation. Results show a clear distinction between growth profiles in the presence and absence of amoxicillin, gentamicin, and fosfomycin, therefore demonstrating a rapid, cost-efficient platform for phenotypic antibiotic susceptibility testing within clinically relevant concentration ranges for conditions such as urinary tract infections and pneumonia.
ORCID iDs
Domingo-Roca, R. ORCID: https://orcid.org/0000-0001-6080-0083, Lasserre, P., Riordan, L., Macdonald, A.R. ORCID: https://orcid.org/0000-0003-2252-3147, Dobrea, A., Duncan, K.R. ORCID: https://orcid.org/0000-0002-3670-4849, Hannah, S., Murphy, M., Hoskisson, P.A. ORCID: https://orcid.org/0000-0003-4332-1640 and Corrigan, D.K. ORCID: https://orcid.org/0000-0002-4647-7483;-
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Item type: Article ID code: 84024 Dates: DateEvent31 May 2023Published3 February 2023Published Online23 January 2023AcceptedSubjects: Medicine > Biomedical engineering. Electronics. Instrumentation Department: Faculty of Engineering > Electronic and Electrical Engineering
Faculty of Engineering > Biomedical Engineering
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > Pure and Applied ChemistryDepositing user: Pure Administrator Date deposited: 06 Feb 2023 15:31 Last modified: 30 Nov 2024 14:22 URI: https://strathprints.strath.ac.uk/id/eprint/84024