Light-sheet mesoscopy with the Mesolens provides fast sub-cellular resolution imaging throughout large tissue volumes

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Battistella, Eliana and Schniete, Jan and Wesencraft, Katrina and Quintana, Juan F. and McConnell, Gail (2022) Light-sheet mesoscopy with the Mesolens provides fast sub-cellular resolution imaging throughout large tissue volumes. iScience, 25 (9). 104797. ISSN 2589-0042 (https://doi.org/10.1016/j.isci.2022.104797)

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Abstract

Rapid imaging of large biological tissue specimens such as ultra thick sections of mouse brain cannot easily be performed with a standard microscope. Optical mesoscopy offers a solution, but thus far imaging has been too slow to be useful for routine use. We have developed two different illuminators for light-sheet mesoscopy with the Mesolens and we demonstrate their use in high-speed optical mesoscale imaging of large tissue specimens. The first light-sheet approach uses Gaussian optics and is straightforward to implement. It provides excellent lateral resolution and high-speed imaging, but the axial resolution is poor. The second light-sheet is a more complex Airy light-sheet that provides sub-cellular resolution in three dimensions that is comparable in quality to point-scanning confocal mesoscopy, but the light-sheet method of illuminating the specimen reduces the imaging time by a factor of 14. This creates new possibilities for high-content, higher-throughput optical bioimaging at the mesoscale.

ORCID iDs

Battistella, Eliana, Schniete, Jan, Wesencraft, Katrina, Quintana, Juan F. and McConnell, Gail ORCID logoORCID: https://orcid.org/0000-0002-7213-0686;
  • Item type:Article
    ID code:81811
    Dates:
    Date
    Event
    16 September 2022
    Published
    21 July 2022
    Published Online
    14 July 2022
    Accepted
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:10 Aug 2022 15:43
    Last modified:17 Dec 2024 01:26
    URI:https://strathprints.strath.ac.uk/id/eprint/81811
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