Selective cytotoxicity of Portuguese Propolis ethyl acetate fraction towards renal cancer cells

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Freitas, Ana Sofia and Costa, Marta and Pontes, Olívia and Seidel, Veronique and Proença, Fernanda and Cardoso, Susana M. and Oliveira, Rui and Baltazar, Fátima and Almeida-Aguiar, Cristina (2022) Selective cytotoxicity of Portuguese Propolis ethyl acetate fraction towards renal cancer cells. Molecules, 27 (13). 4001. ISSN 1420-3049 (https://doi.org/10.3390/molecules27134001)

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Abstract

Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (n-hexane, ethyl acetate, n-butanol and water) and three subfractions (P1–P3), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC50 = 0.162 µg mL−1 ) and 786-O (IC50 = 0.271 µg mL−1 ) cells. With similar toxicity against 786-O, P1 (IC50 = 3.8 µg mL−1 ) and P3 (IC50 = 3.1 µg mL−1 ) exhibited greater effect when combined (IC50 = 2.5 µg mL−1 ). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment.

ORCID iDs

Freitas, Ana Sofia, Costa, Marta, Pontes, Olívia, Seidel, Veronique ORCID logoORCID: https://orcid.org/0000-0003-3880-5261, Proença, Fernanda, Cardoso, Susana M., Oliveira, Rui, Baltazar, Fátima and Almeida-Aguiar, Cristina;
CORE (COnnecting REpositories)