EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes
Koester, Anna M. and Geiser, Angéline and Laidlaw, Kamilla M.E. and Morris, Silke and Cutiongco, Marie F.A. and Stirrat, Laura and Gadegaard, Nikolaj and Boles, Eckhard and Black, Hannah L. and Bryant, Nia J. and Gould, Gwyn W. (2022) EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes. Bioscience Reports, 42 (7). pp. 1-13. BSR20221181. ISSN 1573-4935 (https://doi.org/10.1042/BSR20221181)
Preview |
Text.
Filename: Koester_etal_BR_2022_EFR3_and_phosphatidylinositol_4_kinase_III_alpha_regulate_insulin_stimulated_glucose_transport.pdf
Final Published Version License: Download (2MB)| Preview |
Abstract
Insulin stimulates glucose transport in muscle and adipocytes. This is achieved by regulated delivery of intracellular glucose transporter (GLUT4)-containing vesicles to the plasmamembrane where they dock and fuse, resulting in increased cell surface GLUT4 levels. Recent work identified a potential further regulatory step, in which insulin increases the dispersal of GLUT4 in the plasmamembrane away from the sites of vesicle fusion. EFR3 is a scaffold protein that facilitates localization of phosphatidylinositol 4-kinase type IIIα to the cell surface. Here we show that knockdown of EFR3 or phosphatidylinositol 4-kinase type IIIα impairs insulin-stimulated glucose transport in adipocytes. Using direct stochastic reconstruction microscopy, we also show that EFR3 knockdown impairs insulin stimulated GLUT4 dispersal in the plasma membrane. We propose that EFR3 plays a previously unidentified role in controlling insulin-stimulated glucose transport by facilitating dispersal of GLUT4 within the plasma membrane.
ORCID iDs
Koester, Anna M., Geiser, Angéline ORCID: https://orcid.org/0000-0002-7108-2440, Laidlaw, Kamilla M.E., Morris, Silke, Cutiongco, Marie F.A., Stirrat, Laura, Gadegaard, Nikolaj, Boles, Eckhard, Black, Hannah L., Bryant, Nia J. and Gould, Gwyn W. ORCID: https://orcid.org/0000-0001-6571-2875;-
-
Item type: Article ID code: 81411 Dates: DateEvent8 July 2022Published23 June 2022Published Online22 June 2022AcceptedSubjects: Science > Microbiology Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 08 Jul 2022 11:11 Last modified: 03 Nov 2024 02:14 URI: https://strathprints.strath.ac.uk/id/eprint/81411