Mimicking bone microenvironment : 2D and 3D in vitro models of human osteoblasts
Yuste, I. and Luciano, F. C. and González-Burgos, E. and Lalatsa, A. and Serrano, D. R. (2021) Mimicking bone microenvironment : 2D and 3D in vitro models of human osteoblasts. Pharmacological Research, 169. 105626. ISSN 1096-1186 (https://doi.org/10.1016/j.phrs.2021.105626)
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Abstract
Understanding the in vitro biology and behavior of human osteoblasts is crucial for developing research models that reproduce closely the bone structure, its functions, and the cell-cell and cell-matrix interactions that occurs in vivo. Mimicking bone microenvironment is challenging, but necessary, to ensure the clinical translation of novel medicines to treat more reliable different bone pathologies. Currently, bone tissue engineering is moving from 2D cell culture models such as traditional culture, sandwich culture, micro-patterning, and altered substrate stiffness, towards more complex 3D models including spheroids, scaffolds, cell sheets, hydrogels, bioreactors, and microfluidics chips. There are many different factors, such cell line type, cell culture media, substrate roughness and stiffness that need consideration when developing in vitro models as they affect significantly the microenvironment and hence, the final outcome of the in vitro assay. Advanced technologies, such as 3D bioprinting and microfluidics, have allowed the development of more complex structures, bridging the gap between in vitro and in vivo models. In this review, past and current 2D and 3D in vitro models for human osteoblasts will be described in detail, highlighting the culture conditions and outcomes achieved, as well as the challenges and limitations of each model, offering a widen perspective on how these models can closely mimic the bone microenvironment and for which applications have shown more successful results.
ORCID iDs
Yuste, I., Luciano, F. C., González-Burgos, E., Lalatsa, A. ORCID: https://orcid.org/0000-0003-4791-7468 and Serrano, D. R.;-
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Item type: Article ID code: 80802 Dates: DateEvent31 July 2021Published1 May 2021Published Online15 April 2021AcceptedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 18 May 2022 15:19 Last modified: 05 Sep 2024 01:13 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/80802