A strong inhibitory effect of heather honey, propolis and medicinal plant extracts on biofilm formation by pathogenic bacteria

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Seidel, Veronique and Efthimiou, Georgios (2019) A strong inhibitory effect of heather honey, propolis and medicinal plant extracts on biofilm formation by pathogenic bacteria. Access Microbiology, 1 (10). ISSN 2516-8290 (https://doi.org/10.1099/acmi.amrmeds2019.po0008)

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Abstract

Introduction: P. aeruginosa and S. aureus are opportunistic pathogens that cause a wide range of infections. Their increasing resistance to antibiotics is a serious concern and making them susceptible to treatments is now more essential than ever. There is a need to discover new biofilm inhibitors to increase the susceptibility of these bacteria to antibiotics. Hypothesis and aims: To evaluate the antibiofilm activity of heather honey, propolis and medicinal plant extracts against P. aeruginosa and S. aureus. Methodology: Determination of optimum biofilm growth was carried out using a time-course assay over 24 h intervals, using P. aeruginosa PA14 and S. aureus NCTC 4135 strains. The inhibitory effects of all extracts were determined by biofilm inhibition assay in 24-well plates, with biofilms stained with crystal violet and de-stained with ethanol:acetone; OD were measured at 550 nm. Planktonic growth was measured at 600 nm and samples from the wells were streaked to determine bactericidal effects. Results: Heather honey extracts inhibited both P. aeruginosa and S. aureus by 68%. At 60 μg/mL, one of the propolis extracts promoted biofilm growth of both pathogens. Two other propolis extracts also promoted growth in P. aeruginosa but inhibited biofilm formation in S. aureus by 76.5% and 13.8%, respectively. Three plant extracts inhibited S. aureus biofilm by 7.5%, 10.2% and 87.6% and inhibited P. aeruginosa by −34.9%, 34.7% and 19.4%, respectively. Conclusion: All samples showed varying biofilm inhibition capabilities, but biofilm formation seemed to be more easily inhibited in S. aureus than in P. aeruginosa.

ORCID iDs

Seidel, Veronique ORCID logoORCID: https://orcid.org/0000-0003-3880-5261 and Efthimiou, Georgios;
CORE (COnnecting REpositories)