Single-molecule photoswitching and localization
van de Linde, Sebastian and Wolter, Steve and Sauer, Markus (2011) Single-molecule photoswitching and localization. Australian Journal of Chemistry, 64 (5). pp. 503-511. ISSN 0004-9425 (https://doi.org/10.1071/CH10284)
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Abstract
Within only a few years super-resolution fluorescence imaging based on single-molecule localization and image reconstruction has attracted considerable interest because it offers a comparatively simple way to achieve a substantially improved optical resolution down to ∼20nm in the image plane. Since super-resolution imaging methods such as photoactivated localization microscopy, fluorescence photoactivation localization microscopy, stochastic optical reconstruction microscopy, and direct stochastic optical reconstruction microscopy rely critically on exact fitting of the centre of mass and the shape of the point-spread-function of isolated emitters unaffected by neighbouring fluorophores, controlled photoswitching or photoactivation of fluorophores is the key parameter for resolution improvement. This review will explain the principles and requirements of single-molecule based localization microscopy, and compare different super-resolution imaging concepts and highlight their strengths and limitations with respect to applications in fixed and living cells with high spatio-temporal resolution.
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Item type: Article ID code: 78008 Dates: DateEvent30 May 2011Published4 November 2010AcceptedSubjects: Science > Chemistry
Science > Physics > Optics. Light
Science > MicrobiologyDepartment: Faculty of Science > Physics Depositing user: Pure Administrator Date deposited: 05 Oct 2021 00:52 Last modified: 11 Nov 2024 13:15 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/78008