Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging
van de Linde, Sebastian and Krstić, Ivan and Prisner, Thomas and Doose, Sören and Heilemann, Mike and Sauer, Markus (2011) Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging. Photochemical and Photobiological Sciences, 10 (4). pp. 499-506. ISSN 1474-905X (https://doi.org/10.1039/c0pp00317d)
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Abstract
Radical ions of organic dyes are highly reactive species and have been studied for decades by transient absorption spectroscopy and pulse radiolysis experiments in oxygen-depleted solution. Here we show by continuous wave EPR, absorption, and fluorescence experiments that the triplet state of rhodamine dyes can be photoreduced by thiols to form stable radical anions in aqueous solution with a lifetime of up to several hours. Our data demonstrate that reduction of the triplet state and photoinduced oxidation of reactive intermediates by oxygen represents a general mechanism for reversible photoswitching of dyes in aqueous thiol-containing solutions highlighting the key role of molecular oxygen for super-resolution fluorescence imaging. Since cells contain the thiol glutathione at millimolar concentrations and reactive oxygen species are formed as side products our findings are of consequence for live cell fluorescence microscopy.
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Item type: Article ID code: 77986 Dates: DateEvent10 December 2011Published15 November 2010AcceptedSubjects: Science > Chemistry > Physical and theoretical chemistry Department: Faculty of Science > Physics Depositing user: Pure Administrator Date deposited: 01 Oct 2021 15:34 Last modified: 11 Nov 2024 13:15 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/77986