Unraveling nanotopography of cell surface receptors

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Franke, Christian and Chum, Tomáš and Kvíčalová, Zuzana and Glatzová, Daniela and Rodriguez, Alvaro and Helmerich, Dominic A. and Frank, Otakar and Brdička, Tomáš and van de Linde, Sebastian and Cebecauer, Marek (2020) Unraveling nanotopography of cell surface receptors. Preprint / Working Paper. bioRxiv, Cold Spring Harbor, NY. (https://doi.org/10.1101/2019.12.23.884460)

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Abstract

Cells communicate with their environment via surface receptors, but nanoscopic receptor organization with respect to complex cell surface morphology remains unclear. This is mainly due to a lack of accessible, robust and high-resolution methods. Here, we present an approach for mapping the topography of receptors at the cell surface with nanometer precision. The method involves coating glass coverslips with glycine, which preserves the fine membrane morphology while allowing immobilized cells to be positioned close to the optical surface. We developed an advanced and simplified algorithm for the analysis of single-molecule localization data acquired in a biplane detection scheme. These advancements enable direct and quantitative mapping of protein distribution on ruffled plasma membranes with near isotropic 3D nanometer resolution. As demonstrated successfully for CD4 and CD45 receptors, the described workflow is a straightforward quantitative technique to study molecules and their interactions at the complex surface nanomorphology of differentiated metazoan cells.

  • Item type:Monograph(Preprint / Working Paper)
    ID code:77943
    Dates:
    Date
    Event
    30 July 2020
    Published
    1 July 2020
    Accepted
    Subjects:Science > Microbiology
    Department:Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:30 Sep 2021 09:03
    Last modified:12 Dec 2024 01:53
    URI:https://strathprints.strath.ac.uk/id/eprint/77943
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