Increased levels of IL-16 in the central nervous system during neuroinflammation are associated with infiltrating immune cells and resident glial cells

Hridi, Shehla U and Barbour, Mark and Wilson, Chelsey and Franssen, Aimee JPM and Harte, Tanith and Bushell, Trevor J and Jiang, Hui-Rong (2021) Increased levels of IL-16 in the central nervous system during neuroinflammation are associated with infiltrating immune cells and resident glial cells. Biology, 10 (6). 472. ISSN 2079-7737

[thumbnail of U-Hridi-etal-Biology-2021-Increased-levels-of-IL-16-in-the-central-nervous-system-during-neuroinflammation]
Preview
Text (U-Hridi-etal-Biology-2021-Increased-levels-of-IL-16-in-the-central-nervous-system-during-neuroinflammation)
U_Hridi_etal_Biology_2021_Increased_levels_of_IL_16_in_the_central_nervous_system_during_neuroinflammation.pdf
Final Published Version
License: Creative Commons Attribution 4.0 logo

Download (2MB)| Preview

    Abstract

    Interleukin (IL)-16, a CD4 + immune cell specific chemoattractant cytokine, has been shown to be involved in the development of multiple sclerosis, an inflammatory demyelinating disease of the central nervous system (CNS). While immune cells such as T cells and macrophages are reported to be the producers of IL-16, the cellular source of IL-16 in the CNS is less clear. This study investigates the correlation of IL-16 expression levels in the CNS with the severity of neuroinflammation and determines the phenotype of cells which produce IL-16 in the CNS of experimental autoimmune encephalomyelitis (EAE) mice. Our data show that IL-16 expression is significantly increased in the brain and spinal cord tissues of EAE mice compared to phosphate buffered saline (PBS) immunised controls. Dual immunofluorescence staining reveals that the significantly increased IL-16 + cells in the CNS lesions of EAE mice are likely to be the CD45 + infiltrating immune cells such as CD4 + or F4/80 + cells and the CNS resident CD11b + microglia and GFAP + astrocytes, but not NeuN+ neurons. Our data suggest cytokine IL-16 is closely involved in EAE pathology as evidenced by its increased expression in the glial and infiltrating immune cells, which impacts the recruitment and activation of CD4 + immune cells in the neuroinflammation.

    ORCID iDs

    Hridi, Shehla U ORCID logoORCID: https://orcid.org/0000-0001-7906-6763, Barbour, Mark ORCID logoORCID: https://orcid.org/0000-0003-1547-1289, Wilson, Chelsey ORCID logoORCID: https://orcid.org/0000-0002-1235-1110, Franssen, Aimee JPM ORCID logoORCID: https://orcid.org/0000-0001-6399-1663, Harte, Tanith, Bushell, Trevor J ORCID logoORCID: https://orcid.org/0000-0003-4145-9670 and Jiang, Hui-Rong;