siRNA delivery to melanoma cells with cationic niosomes

Obeid, Mohammad A. and Alyamani, Hanin and Amawi, Haneen and Aljabali, Alaa A. A. and Rezigue, Meriem and Abdeljaber, Shatha N. and Ferro, Valerie A.; Hargadon, K.M., ed. (2021) siRNA delivery to melanoma cells with cationic niosomes. In: Melanoma. Methods in Molecular Biology . Humana Press, New York, pp. 621-634. ISBN 9781071612057 (

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RNA interference (RNAi) is a posttranscriptional regulatory mechanism that employs siRNA. It typically results in the degradation of a target mRNA that encodes a particular protein. Treatment with siRNA therapeutics requires the use of an effective drug delivery system to assist in delivering these therapeutics into the cytoplasm of the transfected cells. Here we describe the transfection of melanoma cancer cells with siRNA using cationic niosome nanoparticles as a delivery system. The method of niosome preparation is first introduced and is followed by complex formation with siRNA and the transfection method.