Population genetics and forensic utility of 23 autosomal PowerPlex Fusion 6C STR loci in the Kuwaiti population

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Haidar, Mahdi and Abbas, Fatimah A. and Alsaleh, Hussain and Haddrill, Penelope R. (2021) Population genetics and forensic utility of 23 autosomal PowerPlex Fusion 6C STR loci in the Kuwaiti population. Scientific Reports, 11. 1865. ISSN 2045-2322 (https://doi.org/10.1038/s41598-021-81425-y)

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Abstract

This study evaluates the forensic utility of 23 autosomal short tandem repeat markers in 400 samples from the Kuwaiti population, of which four markers (D10S1248, D22S1045, D2S441 and SE33) are reported for the first time for Kuwait. All the markers were shown to exhibit no deviation from Hardy–Weinberg equilibrium, nor any linkage disequilibrium between and within loci, indicating that these loci are inherited independently, and their allele frequencies can be used to estimate match probabilities in the Kuwaiti population. The low combined match probability of 7.37 × 10–30 and the high paternity indices generated by these loci demonstrate the usefulness of the PowerPlex Fusion 6C kit for human identification in this population, as well as to strengthen the power of paternity testing. Off-ladder alleles were seen at several loci, and these were identified by examining their underlying nucleotide sequences. Principal component analysis (PCA) and STRUCTURE showed no genetic structure within the Kuwaiti population. However, PCA revealed a correlation between geographic and genetic distance. Finally, phylogenetic trees demonstrated a close relationship between Kuwaitis and Middle Easterners at a global level, and a recent common ancestry for Kuwait with its northern neighbours of Iraq and Iran, at a regional level.

ORCID iDs

Haidar, Mahdi, Abbas, Fatimah A., Alsaleh, Hussain ORCID logoORCID: https://orcid.org/0000-0001-8198-1645 and Haddrill, Penelope R. ORCID logoORCID: https://orcid.org/0000-0001-6805-0694;
  • Item type:Article
    ID code:75155
    Dates:
    Date
    Event
    21 January 2021
    Published
    16 December 2020
    Accepted
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:26 Jan 2021 13:57
    Last modified:15 Dec 2024 01:32
    URI:https://strathprints.strath.ac.uk/id/eprint/75155
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