Label-free molecular detection of antibiotic susceptibility for Mycobacterium smegmatis using a low cost electrode format

Dogan Guzel, Fatma and Ghorbanpoor, Hamed and Norouz Dizaji, Araz and Akcakoca, Iremnur and Ozturk, Yasin and Kocagoz, Tanil and Corrigan, Damion K and Avci, Huseyin (2020) Label-free molecular detection of antibiotic susceptibility for Mycobacterium smegmatis using a low cost electrode format. Biotechnology and Applied Biochemistry. ISSN 1470-8744

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    Abstract

    Today, the emergence of antibiotic resistance in pathogenic bacteria is considered an important problem for society. Excessive consumption of antibiotics, long-term treatments, and inappropriate prescriptions continually increase the severity of the problem. Improving antibiotic stewardship requires improved diagnostic testing, and, therefore, in vitro antibiotic susceptibility testing is becoming increasingly important. This research details the development of an antibiotic susceptibility test for Mycobacterium smegmatis using streptomycin as antibiotics. This strain was selected because it is a member of the slow growing Mycobacterium genus and serves as a useful surrogate organism for M. tuberculosis. A commercially available and low-cost screen-printed gold electrode in combination with a specifically developed nucleic acid probe sequence for the 16SrRNA region of the mycobacterial genome was employed to monitor M. smegmatis nucleic acid sequences using the techniques of square-wave voltammetry and electrochemical impedance spectroscopy. The results show that it was possible to detect M. smegmatis sequences and distinguish antibiotic-treated cells from untreated cells with a label-free molecular detection. As a result, the in vitro antibiotic susceptibility test revealed that M. smegmatis showed sensitivity to streptomycin after a 24-H incubation, with the developed protocol representing a potential approach to determining antibiotic susceptibility more quickly and economically than current methods.

    ORCID iDs

    Dogan Guzel, Fatma, Ghorbanpoor, Hamed, Norouz Dizaji, Araz, Akcakoca, Iremnur, Ozturk, Yasin, Kocagoz, Tanil, Corrigan, Damion K ORCID logoORCID: https://orcid.org/0000-0002-4647-7483 and Avci, Huseyin;
    • Item type:Article
      ID code:74045
      Dates:
      Date
      Event
      25 September 2020
      Published
      25 September 2020
      Published Online
      17 September 2020
      Accepted
      Keywords:antibiotic susceptibility, electrochemical biosensors, antimicrobial resistance (AMR), electrochemical impedance spectroscopy (EIS), square‐wave voltammetry (SWV), Bioengineering, Biomedical Engineering, Applied Microbiology and Biotechnology, Bioengineering, Drug Discovery, Molecular Medicine, Process Chemistry and Technology, Biotechnology
      Subjects:Technology > Engineering (General). Civil engineering (General) > Bioengineering
      Department:Faculty of Engineering > Biomedical Engineering
      Depositing user: Pure Administrator
      Date deposited:01 Oct 2020 15:35
      Last modified:21 Jan 2021 12:23
      URI:https://strathprints.strath.ac.uk/id/eprint/74045
    CORE (COnnecting REpositories)