Ultrasound and microbubbles promote the retention of fluorescent compounds in the small intestine

Turcanu, Mihnea V. and Stewart, Fraser R. and Cox, Ben F. and Clutton, R. Eddie and Mulvana, Helen and Vllasaliu, Driton and Thanou, Maya and Nathke, Inke and Cochran, Sandy; (2018) Ultrasound and microbubbles promote the retention of fluorescent compounds in the small intestine. In: 2018 IEEE International Ultrasonics Symposium, IUS 2018. IEEE International Ultrasonics Symposium, IUS . IEEE Computer Society Press, JPN. ISBN 9781538634257 (https://doi.org/10.1109/ULTSYM.2018.8580036)

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Abstract

Focused ultrasound (US) is a novel means to increase the passage of medication through the wall of the small intestine. The purpose of this study was to determine whether US and microbubbles (MBs) can facilitate delivery of macromolecular therapeutic agents across the intestinal epithelium in vitro and in vivo. In vitro experiments involved delivery of compounds across a cell monolayer, namely Caco-2 cells cultured on ThinCert filters. The cells were cultured for a minimum of 3 weeks to mimic the polarised intestinal epithelium. A suspension of dextran with or without MBs, prepared in growth medium, was introduced into the apical chamber of the ThinCert with a syringe pump through a channel in the centre of a miniature focused US transducer (4 MHz, 1 MPa PNP). Each in vivo experiment involved a tethered endoscopic capsule with an US transducer and a delivery channel inserted into the small intestine of a terminally anaesthetised pig via a surgical stoma. The amount of fluorescent dextran delivered across the Caco-2 monolayer when employing US, MBs and dextran was higher than the amount delivered with dextran alone. With this approach, fluorescent marking of the wall of the small intestine was achieved in vivo by applying US and MBs. Our work indicates that US has potential for application in targeted treatment of gastrointestinal disease and oral drug delivery.

ORCID iDs

Turcanu, Mihnea V., Stewart, Fraser R., Cox, Ben F., Clutton, R. Eddie, Mulvana, Helen ORCID logoORCID: https://orcid.org/0000-0002-5058-0299, Vllasaliu, Driton, Thanou, Maya, Nathke, Inke and Cochran, Sandy;
  • Item type:Book Section
    ID code:71718
    Dates:
    Date
    Event
    20 December 2018
    Published
    13 July 2018
    Accepted
    Notes:© 2018 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting /republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
    Subjects:Technology > Engineering (General). Civil engineering (General) > Bioengineering
    Department:Faculty of Engineering > Biomedical Engineering
    Depositing user: Pure Administrator
    Date deposited:06 Mar 2020 12:11
    Last modified:11 Nov 2024 15:20
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/71718
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