A controlled in vitro study of optimal low intensity pulsed ultrasound fields for stimulation of proliferation in murine osteoblasts

Savva, Jill and Lucas, Margaret and Mulvana, Helen; (2019) A controlled in vitro study of optimal low intensity pulsed ultrasound fields for stimulation of proliferation in murine osteoblasts. In: 2019 IEEE International Ultrasonics Symposium, IUS 2019. IEEE International Ultrasonics Symposium, IUS . IEEE Computer Society Press, GBR, pp. 1543-1546. ISBN 9781728145969 (https://doi.org/10.1109/ULTSYM.2019.8925594)

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Abstract

Clinical, in vivo and in vitro studies have established that Low Intensity Pulsed Ultrasound (LIPUS) stimulates healing of fractured bone, but the mechanisms are not well understood. In vitro studies show cell proliferation, migration and many cellular markers are stimulated by LIPUS at frequencies of 1.0 - 1.5 MHz, even down to 45 kHz [1]. However, most trials did not control or measure the acoustic field, so the dose experienced by the cells across such studies cannot be compared. An in vitro ultrasound exposure method was developed to maintain control of the acoustic field. Murine osteoblasts (MC3T3-E1) were exposed to 20-minute LIPUS fields at the frequencies 1 MHz and 45 kHz and Mechanical Index from 0 (control) to 0.2. Cell proliferation was assessed by counting viable cells immediately before and twenty hours after LIPUS exposure in the centre of a custom-designed cell culture vessel. Initial results indicate that LIPUS fields increase cell proliferation at 1 MHz, 0.1 MI and more significantly at 45 kHz, 0.2 MI compared to controls, but has a detrimental or no effect otherwise. Future work will involve further repeats to acquire larger data sets, along with cell counts in other areas of the cell growth surface to increase data obtained from a single repeat. The study demonstrates the efficacy of the method for quantitative in vitro investigation of LPUS mechanisms and will be used in future work to find optimum LIPUS field characteristics, in terms of frequency, Mechanical Index, and pulse characteristics such as pulse repetition rate and pulse width.

ORCID iDs

Savva, Jill, Lucas, Margaret and Mulvana, Helen ORCID logoORCID: https://orcid.org/0000-0002-5058-0299;
  • Item type:Book Section
    ID code:71714
    Dates:
    Date
    Event
    9 December 2019
    Published
    6 October 2019
    Accepted
    Notes:© 2019 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting /republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
    Subjects:Technology > Engineering (General). Civil engineering (General) > Bioengineering
    Department:Faculty of Engineering > Biomedical Engineering
    Depositing user: Pure Administrator
    Date deposited:06 Mar 2020 11:39
    Last modified:11 Nov 2024 15:20
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/71714
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