Establishing a field-effect transistor sensor for the detection of mutations in the tumour protein 53 gene (TP53) : an electrochemical optimisation approach

Crossley, Lisa and Attoye, Bukola and Vezza, Vincent and Blair, Ewen and Corrigan, Damion K. and Hannah, Stuart (2019) Establishing a field-effect transistor sensor for the detection of mutations in the tumour protein 53 gene (TP53) : an electrochemical optimisation approach. Biosensors, 9 (4). 141. ISSN 2079-6374

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    Abstract

    We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and non-complementary DNA target sequences.