Establishing a field-effect transistor sensor for the detection of mutations in the tumour protein 53 gene (TP53) : an electrochemical optimisation approach

Crossley, Lisa and Attoye, Bukola and Vezza, Vincent and Blair, Ewen and Corrigan, Damion K. and Hannah, Stuart (2019) Establishing a field-effect transistor sensor for the detection of mutations in the tumour protein 53 gene (TP53) : an electrochemical optimisation approach. Biosensors, 9 (4). 141. ISSN 2079-6374

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    Abstract

    We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and non-complementary DNA target sequences.

    Author(s):Crossley, Lisa, Attoye, Bukola, Vezza, Vincent, Blair, Ewen ORCID logoORCID: https://orcid.org/0000-0002-1887-8001, Corrigan, Damion K. ORCID logoORCID: https://orcid.org/0000-0002-4647-7483 and Hannah, Stuart ORCID logoORCID: https://orcid.org/0000-0001-5620-9899
    Item type:Article
    ID code:70728
    Keywords:field-effect transistor, biosensor, TP53, electrochemistry, open-circuit potential, Bioengineering, Chemical Engineering(all), Biophysics, Biomedical Engineering
    Subjects:Technology > Engineering (General). Civil engineering (General) > Bioengineering
    Department:Faculty of Engineering > Biomedical Engineering
    Depositing user: Pure Administrator
    Date deposited:06 Dec 2019 14:29
    Last modified:16 Jan 2020 04:18
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/70728
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