Freeze-drying cycle optimization for the rapid preservation of protein-loaded liposomal formulations

Hussain, Maryam T and Forbes, Neil and Perrie, Yvonne and Malik, Kiran P and Duru, Chinwe and Matejtschuk, Paul (2020) Freeze-drying cycle optimization for the rapid preservation of protein-loaded liposomal formulations. International Journal of Pharmaceutics, 573. pp. 1-10. 118722. ISSN 1873-3476 (https://doi.org/10.1016/j.ijpharm.2019.118722)

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Abstract

Technology such as the use of microfluidics to generate liposomes has been well researched, yet the stabilisation of liposomal formulations is a major challenge to their greater implementation. To the best of our knowledge, this is the first study investigating the use of 96 well plates to freeze-dry ovalbumin (OVA) loaded neutral (DMPC:Chol and DSPC:Chol), anionic (DSPC:Chol:PS) and cationic (DSPC:Chol:DOTAP) liposomes. Through the use of high throughput screening, a freeze drying cycle was optimised; ramp freezing from from 4°C to -45°C, followed by primary drying at -30°C and secondary drying at 30°C under a vacuum of 0.1 mBar. These parameters maintained liposome physicochemical properties, with the liposomes remaining below 100 nm and were homogenous (polydispersity index of less than 0.2 post rehydration). Minimal leakage of the OVA protein was observed, with almost 100% OVA remaining encapsulated post rehydration of the formulations. Here we have identified a simple method that allows for the rapid screening and freeze-drying of a range of liposomal formulations.

  • Item type:Article
    ID code:70048
    Dates:
    Date
    Event
    5 January 2020
    Published
    6 November 2019
    Published Online
    19 September 2019
    Accepted
    28 June 2019
    Submitted
    Notes:© 2019 Published by Elsevier B.V. All rights reserved Maryam T. Hussain, Neil Forbes, Yvonne Perrie, Kiran P. Malik, Chinwe Duru, Paul Matejtschuk, Freeze-drying cycle optimization for the rapid preservation of protein-loaded liposomal formulations, International Journal of Pharmaceutics, Volume 573, 2020, 118722, https://doi.org/10.1016/j.ijpharm.2019.118722
    Subjects:Medicine > Therapeutics. Pharmacology
    Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
    Depositing user: Pure Administrator
    Date deposited:08 Oct 2019 08:42
    Last modified:06 Nov 2024 01:48
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/70048
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