European propolis is highly active against trypanosomatids including Crithidia fasciculata

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Alotaibi, Abdullah and Ebiloma, Godwin U. and Williams, Roderick and Alenezi, Samya and Donachie, Anne-Marie and Guillaume, Selome and Igoli, John O. and Fearnley, James and de Koning, Harry P. and Watson, David G. (2019) European propolis is highly active against trypanosomatids including Crithidia fasciculata. Scientific Reports, 9 (1). 11364. ISSN 2045-2322 (https://doi.org/10.1038/s41598-019-47840-y)

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Abstract

Extracts of 35 samples of European propolis were tested against wild type and resistant strains of the protozoal pathogens Trypanosoma brucei, Trypanosoma congolense and Leishmania mexicana. The extracts were also tested against Crithidia fasciculata a close relative of Crithidia mellificae, a parasite of bees. Crithidia, Trypanosoma and Leishmania are all members of the order Kinetoplastida. High levels of activity were obtained for all the samples with the levels of activity varying across the sample set. The highest levels of activity were found against L. mexicana. The propolis samples were profiled by using liquid chromatography with high resolution mass spectrometry (LC-MS) and principal components analysis (PCA) of the data obtained indicated there was a wide variation in the composition of the propolis samples. Orthogonal partial least squares (OPLS) associated a butyrate ester of pinobanksin with high activity against T. brucei whereas in the case of T. congolense high activity was associated with methyl ethers of chrysin and pinobanksin. In the case of C. fasciculata highest activity was associated with methyl ethers of galangin and pinobanksin. OPLS modelling of the activities against L. mexicana using the mass spectrometry produced a less successful model suggesting a wider range of active components.

ORCID iDs

Alotaibi, Abdullah, Ebiloma, Godwin U., Williams, Roderick, Alenezi, Samya, Donachie, Anne-Marie, Guillaume, Selome, Igoli, John O., Fearnley, James, de Koning, Harry P. and Watson, David G. ORCID logoORCID: https://orcid.org/0000-0003-1094-7604;
CORE (COnnecting REpositories)