Investigating pre-analytical requirements for serum and plasma based infrared spectro-diagnostic
Lovergne, Lila and Lovergne, Jean and Bouzy, Pascaline and Untereiner, Valérie and Offroy, Marc and Garnotel, Roselyne and Thiéfin, Gérard and Baker, Matthew J. and Sockalingum, Ganesh D. (2019) Investigating pre-analytical requirements for serum and plasma based infrared spectro-diagnostic. Journal of Biophotonics, 12 (12). e201900177. ISSN 1864-0648 (https://doi.org/10.1002/jbio.201900177)
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Abstract
Infrared spectroscopy is a rapid, easy‐to‐operate, label‐free and therefore cost‐effective technique. Many studies performed on biofluids (e.g. serum, plasma, urine, sputum, bile, cerebrospinal fluid) have demonstrated its promising application as a clinical diagnostic tool. Given all these characteristics, infrared spectroscopy appears to be an ideal candidate to be implemented into the clinics. However, before considering its translation, a clear effort is needed to standardise protocols for biofluid spectroscopic analysis. To reach this goal, careful investigations to identify and track errors that can occur during the pre‐analytical phase is a crucial step. Here, we report for the first time, results of investigations into pre‐analytical factors that can affect the quality of the spectral data acquired on serum and plasma, such as the impact of long‐term freezing time storage of samples as well as the month‐ to‐month reproducibility of the spectroscopic analysis. The spectral data discrimination has revealed to be majorly impacted by a residual water content variation in serum and plasma dried samples.
ORCID iDs
Lovergne, Lila, Lovergne, Jean, Bouzy, Pascaline, Untereiner, Valérie, Offroy, Marc, Garnotel, Roselyne, Thiéfin, Gérard, Baker, Matthew J. ORCID: https://orcid.org/0000-0003-2362-8581 and Sockalingum, Ganesh D.;-
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Item type: Article ID code: 68811 Dates: DateEvent31 December 2019Published5 July 2019Published Online4 July 2019AcceptedSubjects: Science > Chemistry Department: Strategic Research Themes > Health and Wellbeing
Faculty of Science > Pure and Applied ChemistryDepositing user: Pure Administrator Date deposited: 11 Jul 2019 10:38 Last modified: 04 Dec 2024 01:21 URI: https://strathprints.strath.ac.uk/id/eprint/68811