Minimalistic supramolecular proteoglycan mimics by co-assembly of aromatic peptide and carbohydrate amphiphiles
Brito, Alexandra and Abul-Haija, Yousef M. and Da Costa, Diana Soares and Novoa-Carballal, Ramon and Reis, Rui L. and Ulijn, Rein V. and Pires, Ricardo A. and Pashkuleva, Iva (2019) Minimalistic supramolecular proteoglycan mimics by co-assembly of aromatic peptide and carbohydrate amphiphiles. Chemical Science, 10 (8). pp. 2385-2390. ISSN 2041-6539 (https://doi.org/10.1039/C8SC04361B)
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Abstract
We report the co-assembly of aromatic carbohydrate and dipeptide amphiphiles under physiological conditions as a strategy to generate minimalistic proteoglycan mimics. The resulting nanofibers present a structural, fluorenylmethoxycarbonyl-diphenylalanine (Fmoc-FF) core and a functional carbohydrate (Fmoc-glucosamine-6-sulfate or -phosphate) shell. The size, degree of bundling and mechanical properties of the assembled structures depend on the chemical nature of the carbohydrate amphiphile used. In cell culture medium, these nanofibers can further organize into supramolecular hydrogels. We demonstrate that, similar to proteoglycans, the assembled gels prolong the stability of growth factors and preserve the viability of cultured cells. Our results demonstrate that this approach can be applied to the design of extracellular matrix (ECM) substitutes for future regenerative therapies.
ORCID iDs
Brito, Alexandra, Abul-Haija, Yousef M. ORCID: https://orcid.org/0000-0002-0357-0653, Da Costa, Diana Soares, Novoa-Carballal, Ramon, Reis, Rui L., Ulijn, Rein V. ORCID: https://orcid.org/0000-0001-7974-3779, Pires, Ricardo A. and Pashkuleva, Iva;-
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Item type: Article ID code: 67400 Dates: DateEvent28 February 2019Published21 December 2018Published Online21 December 2018AcceptedSubjects: Science > Chemistry Department: Faculty of Science > Pure and Applied Chemistry Depositing user: Pure Administrator Date deposited: 21 Mar 2019 11:43 Last modified: 29 Aug 2024 00:50 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/67400