Effects of silk degumming process on physicochemical, tensile, and optical properties of regenerated silk fibroin
Nultsch, Kira and Bast, Livia K. and Näf, Muriel and Yakhlifi, Salima El and Bruns, Nico and Germershaus, Oliver (2018) Effects of silk degumming process on physicochemical, tensile, and optical properties of regenerated silk fibroin. Macromolecular Materials and Engineering, 303 (12). 1800408. ISSN 1439-2054 (https://doi.org/10.1002/mame.201800408)
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Abstract
Sericin removal from silk (degumming) affects material characteristics of silk fibroin (SF). Sodium carbonate is most commonly used for degumming, but numerous alternative methods are available. Herein, a systematic comparison of degumming methods is provided. Sodium carbonate, sodium oleate, trypsin, and ionic liquid are used, and materials are characterized regarding mass loss, SF content, molecular integrity of SF, refractive index, and tensile properties. Complete degumming is achieved within 30 min of using sodium carbonate, but results in significant reduction of molecular weight, shift toward less acidic charge variants, and reduction of yield- and rupture force. Sodium oleate and trypsin are inefficient and negatively affect tensile properties, while ionic liquid shows good efficiency and marginal degradation of SF but also reduced yield- and rupture force. Refractive index is not affected by degumming. These results allow rational selection of the degumming method and tuning of SF properties for biomedical applications.
ORCID iDs
Nultsch, Kira, Bast, Livia K., Näf, Muriel, Yakhlifi, Salima El, Bruns, Nico ORCID: https://orcid.org/0000-0001-6199-9995 and Germershaus, Oliver;-
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Item type: Article ID code: 66930 Dates: DateEvent31 December 2018Published21 September 2018Published Online27 August 2018AcceptedSubjects: Science > Chemistry Department: Faculty of Science > Pure and Applied Chemistry Depositing user: Pure Administrator Date deposited: 12 Feb 2019 16:51 Last modified: 24 Oct 2024 00:32 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/66930