Metabolomic-guided isolation of bioactive natural products from Curvularia sp., an endophytic fungus of Terminalia laxiflora
Tawfike, Ahmed F. and Abbott, Grainne and Young, Louise and Edrada-Ebel, Ruangelie (2018) Metabolomic-guided isolation of bioactive natural products from Curvularia sp., an endophytic fungus of Terminalia laxiflora. Planta Medica, 84 (3). pp. 182-190. ISSN 0032-0943 (https://doi.org/10.1055/s-0043-118807)
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Abstract
Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology. Metabolomic tools were successfully employed to compare the metabolite fingerprints of solid and liquid culture extracts of endophyte Curvularia sp. isolated from the leaves of Terminalia laxiflora. Natural product databases were used to dereplicate metabolites in order to determine known compounds and the presence of new natural products. Multivariate analysis highlighted the putative metabolites responsible for the bioactivity of the fungal extract and its fractions on NF-kappaB and the myelogenous leukemia cell line K562. Metabolomic tools and dereplication studies using HRESIMS directed the fractionation and isolation of the bioactive components from the fungal extracts. This resulted in the isolation of N-acetylphenylalanine (1) and two linear peptide congeners of 1: dipeptide N-acetylphenylalanyl-L-phenylalanine (2) and tripeptide N-acetylphenylalanyl-L-phenylalanyl-L-leucine (3).
ORCID iDs
Tawfike, Ahmed F. ORCID: https://orcid.org/0000-0003-2061-3093, Abbott, Grainne, Young, Louise and Edrada-Ebel, Ruangelie;-
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Item type: Article ID code: 65165 Dates: DateEvent28 February 2018Published28 August 2017Published Online21 August 2017AcceptedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 14 Aug 2018 15:33 Last modified: 01 Sep 2024 01:16 URI: https://strathprints.strath.ac.uk/id/eprint/65165