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Metabolomic-guided isolation of bioactive natural products from Curvularia sp., an endophytic fungus of Terminalia laxiflora

Tawfike, Ahmed F. and Abbott, Grainne and Young, Louise and Edrada-Ebel, Ruangelie (2018) Metabolomic-guided isolation of bioactive natural products from Curvularia sp., an endophytic fungus of Terminalia laxiflora. Planta Medica, 84 (3). pp. 182-190. ISSN 0032-0943

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Abstract

Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology. Metabolomic tools were successfully employed to compare the metabolite fingerprints of solid and liquid culture extracts of endophyte Curvularia sp. isolated from the leaves of Terminalia laxiflora. Natural product databases were used to dereplicate metabolites in order to determine known compounds and the presence of new natural products. Multivariate analysis highlighted the putative metabolites responsible for the bioactivity of the fungal extract and its fractions on NF-kappaB and the myelogenous leukemia cell line K562. Metabolomic tools and dereplication studies using HRESIMS directed the fractionation and isolation of the bioactive components from the fungal extracts. This resulted in the isolation of N-acetylphenylalanine (1) and two linear peptide congeners of 1: dipeptide N-acetylphenylalanyl-L-phenylalanine (2) and tripeptide N-acetylphenylalanyl-L-phenylalanyl-L-leucine (3).