NADPH as a potential intrinsic probe for tumour margin estimation

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Stewart, Hazel L. and Hupp, Ted R. and Birch, David J. S.; Pogue, Brian W. and Gioux, Sylvain and Biggs, Gregg, eds. (2018) NADPH as a potential intrinsic probe for tumour margin estimation. In: Molecular-Guided Surgery. Proceedings of SPIE . SPIE, USA. (https://doi.org/10.1117/12.2287414)

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Abstract

The fluorescent properties of the reduced coenzyme NADH and its phosphorylated derivative (NADPH) have been explored in order to assess their potential as an intrinsic probe for cancer surgery. NADPH production is increased in cancer cells to quench reactive oxygen species and meet higher demands for biosynthesis, and has attractive fluorescent properties such as emission towards the visible part of the spectrum and a relatively long fluorescence lifetime upon binding to enzymes (~ 1 – 6.5 ns) that helps discriminate against other endogenous species. Different environmental effects on NAD(P)H fluorescence are reported here, including an increase in lifetime upon oxygen removal, an ability to retain its fluorescent properties in a complex medium (a silica phantom) and its fluorescence lifetime also being distinguishable in a cell environment. In addition, the development of a miniaturized liquid light guide filter-based timecorrelated single photon counting fluorescence lifetime system is reported as a step towards time-resolved visual imaging in cancer surgery. This system has been demonstrated as being capable of accurately measuring NAD(P)H fluorescence lifetimes in both simple solvent and cellular environments.

ORCID iDs

Stewart, Hazel L., Hupp, Ted R. and Birch, David J. S. ORCID logoORCID: https://orcid.org/0000-0001-6400-1270; Pogue, Brian W., Gioux, Sylvain and Biggs, Gregg
  • Item type:Book Section
    ID code:63575
    Dates:
    Date
    Event
    1 March 2018
    Published
    25 September 2017
    Accepted
    Notes:Hazel Stewart, Ted R. Hupp, David J. S. Birch, "NADPH as a potential intrinsic probe for tumour margin estimation", Proc. SPIE 10478, Molecular-Guided Surgery: Molecules, Devices, and Applications IV, 1047817 (1 March 2018); doi: 10.1117/12.2287414. © 2018 Society of Photo Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, or modification of the contents of the publication are prohibited.
    Subjects:Science > Physics
    Department:Technology and Innovation Centre > Bionanotechnology
    Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:03 Apr 2018 14:18
    Last modified:23 Nov 2024 01:25
    URI:https://strathprints.strath.ac.uk/id/eprint/63575
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