Detecting beta-amyloid aggregation from the time-resolved emission spectra

Alghamdi, Abeer and Vyshemirsky, Vladislav and Birch, David J S and Rolinski, Olaf J (2017) Detecting beta-amyloid aggregation from the time-resolved emission spectra. Methods and Applications in Fluorescence. ISSN 2050-6120 (https://doi.org/10.1088/2050-6120/aa9f95)

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Abstract

The aggregation of beta-amyloids is one of the key processes responsible for the development of Alzheimer's disease. Early molecular-level detection of beta-amyloid oligomers may help in early diagnosis and in the development of new intervention therapies. Our previous studies on the changes in beta-amyloid's single tyrosine intrinsic fluorescence response during aggregation demonstrated a four-exponential fluorescence intensity decay, and the ratio of the pre-exponential factors indicated the extent of the aggregation in the early stages of the process before the beta-sheets were formed. Here we present a complementary approach based on the time-resolved emission spectra (TRES) of amyloid's tyrosine excited at 279 nmand fluorescent in the window 240–450 nm. TRES has been used to demonstrate sturctural changes occuring on the nanosecond time scale after excitation which has significant advantages over using steady-state spectra.Wedemonstrate this by resolving the fluorescent species and revealing that beta-amyloid's monomers show very fast dielectric relaxation, and its oligomers display a substantial spectral shift due to dielectric relaxation, which gradually decreases when the oligomers become larger.

ORCID iDs

Alghamdi, Abeer, Vyshemirsky, Vladislav, Birch, David J S ORCID logoORCID: https://orcid.org/0000-0001-6400-1270 and Rolinski, Olaf J ORCID logoORCID: https://orcid.org/0000-0002-7838-779X;
  • Item type:Article
    ID code:62890
    Dates:
    Date
    Event
    6 December 2017
    Published
    6 December 2017
    Published Online
    6 December 2017
    Accepted
    Notes:This is an author-created, un-copyedited version of an article accepted for publication in Methods and Applications in Fluorescence . IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.1088/2050-6120/aa9f95.
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Technology and Innovation Centre > Bionanotechnology
    Depositing user: Pure Administrator
    Date deposited:16 Jan 2018 11:50
    Last modified:17 Nov 2024 18:39
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/62890
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