Insulin aggregation tracked by its intrinsic TRES

Chung, Li Hung C. and Birch, David J. S. and Vyshemirsky, Vladislav and Ryadnov, Maxim G. and Rolinski, Olaf J. (2017) Insulin aggregation tracked by its intrinsic TRES. Applied Physics Letters, 111. ISSN 0003-6951 (

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Time-resolved emission spectra (TRES) have been used to detect conformational changes of intrinsic tyrosines within bovine insulin at a physiological pH. The approach offers the ability to detect the initial stages of insulin aggregation at the molecular level. The data analysis has revealed the existence of at least three fluorescent species undergoing dielectric relaxation and significant spectral changes due to insulin aggregation. The results indicate suitability of the intrinsic TRES approach for insulin studies and for monitoring its stability during storage and aggregation in insulin delivery devices.