Insulin aggregation tracked by its intrinsic TRES

Chung, Li Hung C. and Birch, David J. S. and Vyshemirsky, Vladislav and Ryadnov, Maxim G. and Rolinski, Olaf J. (2017) Insulin aggregation tracked by its intrinsic TRES. Applied Physics Letters, 111. ISSN 0003-6951 (https://doi.org/10.1063/1.5008477)

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Abstract

Time-resolved emission spectra (TRES) have been used to detect conformational changes of intrinsic tyrosines within bovine insulin at a physiological pH. The approach offers the ability to detect the initial stages of insulin aggregation at the molecular level. The data analysis has revealed the existence of at least three fluorescent species undergoing dielectric relaxation and significant spectral changes due to insulin aggregation. The results indicate suitability of the intrinsic TRES approach for insulin studies and for monitoring its stability during storage and aggregation in insulin delivery devices.

ORCID iDs

Chung, Li Hung C., Birch, David J. S. ORCID logoORCID: https://orcid.org/0000-0001-6400-1270, Vyshemirsky, Vladislav, Ryadnov, Maxim G. and Rolinski, Olaf J. ORCID logoORCID: https://orcid.org/0000-0002-7838-779X;
  • Item type:Article
    ID code:62887
    Dates:
    Date
    Event
    27 December 2017
    Published
    27 December 2017
    Published Online
    8 December 2017
    Accepted
    Notes:This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. The following article appeared in Chung, LHC, Birch, DJS, Vyshemirsky, V, Ryadnov, MG & Rolinski, OJ 2017, 'Insulin aggregation tracked by its intrinsic TRES' Applied Physics Letters, vol 111 and may be found at https://doi.org/10.1063/1.5008477.
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Technology and Innovation Centre > Bionanotechnology
    Depositing user: Pure Administrator
    Date deposited:16 Jan 2018 11:27
    Last modified:11 Nov 2024 11:53
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/62887
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