Photometry unlocks 3D information from 2D localization microscopy data
Franke, Christian and Sauer, Markus and van de Linde, Sebastian (2017) Photometry unlocks 3D information from 2D localization microscopy data. Nature Methods, 14 (1). pp. 41-44. ISSN 1548-7091
|
Text (Franke-etal-NM-2017-Photometry-unlocks-3D-information-from-2D-localization-microscopy)
Franke_etal_NM_2017_Photometry_unlocks_3D_information_from_2D_localization_microscopy.pdf Accepted Author Manuscript Download (180kB)| Preview |
Abstract
We developed a straightforward photometric method, temporal, radial-aperture-based intensity estimation (TRABI), that allows users to extract 3D information from existing 2D localization microscopy data. TRABI uses the accurate determination of photon numbers in different regions of the emission pattern of single emitters to generate a z-dependent photometric parameter. This method can determine fluorophore positions up to 600 nm from the focal plane and can be combined with biplane detection to further improve axial localization.
Author(s): | Franke, Christian, Sauer, Markus and van de Linde, Sebastian | Item type: | Article |
---|---|
ID code: | 61645 |
Keywords: | super resolution, microscopy, photometry, Physics, Biochemistry, Cell Biology, Molecular Biology, Biotechnology |
Subjects: | Science > Physics |
Department: | Faculty of Science > Physics |
Depositing user: | Pure Administrator |
Date deposited: | 28 Aug 2017 11:26 |
Last modified: | 07 Dec 2019 04:27 |
URI: | https://strathprints.strath.ac.uk/id/eprint/61645 |
Export data: |