Photometry unlocks 3D information from 2D localization microscopy data

Franke, Christian and Sauer, Markus and van de Linde, Sebastian (2017) Photometry unlocks 3D information from 2D localization microscopy data. Nature Methods, 14 (1). pp. 41-44. ISSN 1548-7105 (https://doi.org/10.1038/nmeth.4073)

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Abstract

We developed a straightforward photometric method, temporal, radial-aperture-based intensity estimation (TRABI), that allows users to extract 3D information from existing 2D localization microscopy data. TRABI uses the accurate determination of photon numbers in different regions of the emission pattern of single emitters to generate a z-dependent photometric parameter. This method can determine fluorophore positions up to 600 nm from the focal plane and can be combined with biplane detection to further improve axial localization.

  • Item type:Article
    ID code:61645
    Dates:
    Date
    Event
    31 January 2017
    Published
    21 November 2016
    Published Online
    16 October 2016
    Accepted
    Keywords:super resolution, microscopy, photometry, Physics, Biochemistry, Cell Biology, Molecular Biology, Biotechnology
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:28 Aug 2017 11:26
    Last modified:02 May 2022 00:21
    URI:https://strathprints.strath.ac.uk/id/eprint/61645
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