Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system

Dimov, Nikolay and Kastner, Elisabeth and Hussain, Maryam Tabassum and Perrie, Yvonne and Szita, Nicolas (2017) Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system. Scientific Reports, 7 (1). ISSN 2045-2322

[img]
Preview
Text (Dimov-etal-SR-2017-Formation-and-purification-of-tailored-liposomes-for-drug-delivery)
Dimov_etal_SR_2017_Formation_and_purification_of_tailored_liposomes_for_drug_delivery.pdf
Accepted Author Manuscript
License: Creative Commons Attribution 4.0 logo

Download (612kB)| Preview

    Abstract

    Liposomes are lipid based bilayer vesicles that can encapsulate, deliver and release low-soluble drugs and small molecules to a specific target site in the body and they are currently exploited in several nanomedicine formulations. However, their development and application is still limited by expensive and time-consuming process development and production methods. Therefore, to exploit these systems more effectively and support the rapid translation of new liposomal nanomedicines from bench to bedside, new cost-effective and scalable production methods are much needed. Here we present a continuous process flow system for the preparation, modification and purification of liposomes which offers lab-on-chip scale production. The system was evaluated for a range of small vesicles (below 300 nm) varying in lipid composition, size and charge. This system offers effective and rapid nanomedicine purification with high lipid recovery (>98%) combined with effective removal of non-entrapped drug (propofol >95% reduction of non-entrapped drug present) or protein (ovalbumin >90% reduction of OVA present) and organic solvent (ethanol >95% reduction) in less than 4 minutes. Within this manuscript, we outline a new set-up that offer the key advantages of using this bench-top, rapid, process development tool are the flexible operating conditions, interchangeable membranes and scalable high-throughput yields, thereby offering simultaneous manufacturing and purification of nanoparticles with tailored surface attributes.