A 340/380 nm light emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision
Tinning, Peter William and Franssen, Aimee and Hridi, Shehla Unaiza and Bushell, Trevor and McConnell, Gail (2017) A 340/380 nm light emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision. In: Microscience Microscopy Congress 2017, 2017-07-03 - 2017-07-06.
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Abstract
Cytosolic Ca2+ plays an integral role in cells and the study of its dynamics can reveal much about biological processes [1]. Fura-2 can provide quantitative data on cytosolic Ca2+ changes by exciting at 340 nm and 380 nm and taking the ratio of the emission at both wavelengths [2]. Traditionally for this type of imaging an arc lamp had to be used for illumination as LEDs of the appropriate wavelengths were not available [3]. LEDs hold advantages over arc lamps by exhibiting high amplitude stability and the ability to rapidly switch between wavelengths. We aimed to test a new 340/380 nm LED system for use in ratiometric Fura-2 AM Ca2+ imaging and present results using tsA-201 cells and hippocampal neurons.
Creators(s): |
Tinning, Peter William ![]() ![]() ![]() ![]() ![]() | Item type: | Conference or Workshop Item(Poster) |
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ID code: | 61503 |
Keywords: | cell imaging, light emitting diode, LED wavelength, Pharmacy and materia medica, Pharmacology, Toxicology and Pharmaceutics(all) |
Subjects: | Medicine > Pharmacy and materia medica |
Department: | Faculty of Science > Physics Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Strategic Research Themes > Measurement Science and Enabling Technologies |
Depositing user: | Pure Administrator |
Date deposited: | 07 Aug 2017 13:27 |
Last modified: | 21 Jan 2021 13:04 |
Related URLs: | |
URI: | https://strathprints.strath.ac.uk/id/eprint/61503 |
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