A 340/380 nm light emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision

Tinning, Peter William and Franssen, Aimee and Hridi, Shehla Unaiza and Bushell, Trevor and McConnell, Gail (2017) A 340/380 nm light emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision. In: Microscience Microscopy Congress 2017, 2017-07-03 - 2017-07-06.

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    Abstract

    Cytosolic Ca2+ plays an integral role in cells and the study of its dynamics can reveal much about biological processes [1]. Fura-2 can provide quantitative data on cytosolic Ca2+ changes by exciting at 340 nm and 380 nm and taking the ratio of the emission at both wavelengths [2]. Traditionally for this type of imaging an arc lamp had to be used for illumination as LEDs of the appropriate wavelengths were not available [3]. LEDs hold advantages over arc lamps by exhibiting high amplitude stability and the ability to rapidly switch between wavelengths. We aimed to test a new 340/380 nm LED system for use in ratiometric Fura-2 AM Ca2+ imaging and present results using tsA-201 cells and hippocampal neurons.

    Creators(s): Tinning, Peter William ORCID logoORCID: https://orcid.org/0000-0002-3270-4120, Franssen, Aimee ORCID logoORCID: https://orcid.org/0000-0001-6399-1663, Hridi, Shehla Unaiza ORCID logoORCID: https://orcid.org/0000-0001-7906-6763, Bushell, Trevor ORCID logoORCID: https://orcid.org/0000-0003-4145-9670 and McConnell, Gail ORCID logoORCID: https://orcid.org/0000-0002-7213-0686;
    Item type:Conference or Workshop Item(Poster)
    ID code:61503
    Keywords:cell imaging, light emitting diode, LED wavelength, Pharmacy and materia medica, Pharmacology, Toxicology and Pharmaceutics(all)
    Subjects:Medicine > Pharmacy and materia medica
    Department:Faculty of Science > Physics
    Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
    Strategic Research Themes > Measurement Science and Enabling Technologies
    Depositing user: Pure Administrator
    Date deposited:07 Aug 2017 13:27
    Last modified:21 Jan 2021 13:04
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/61503
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