Errors in the measurement of voltage-activated ion channels in cell-attached patch-clamp recordings
Williams, Stephen R. and Wozny, Christian (2011) Errors in the measurement of voltage-activated ion channels in cell-attached patch-clamp recordings. Nature Communications, 2. 242. ISSN 2041-1723 (https://doi.org/10.1038/ncomms1225)
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Abstract
Patch-clamp recording techniques have revolutionized understanding of the function and sub-cellular location of ion channels in excitable cells. The cell-attached patch-clamp configuration represents the method of choice to describe the endogenous properties of voltage-activated ion channels in the axonal, somatic and dendritic membrane of neurons, without disturbance of the intracellular milieu. Here, we directly examine the errors associated with cell-attached patch-clamp measurement of ensemble ion channel activity. We find for a number of classes of voltage-activated channels, recorded from the soma and dendrites of neurons in acute brain-slices and isolated cells, that the amplitude and kinetics of ensemble ion channel activity recorded in cell-attached patches is significantly distorted by transmembrane voltage changes generated by the flow of current through the activated ion channels. We outline simple error-correction procedures that allow a more accurate description of the density and properties of voltage-activated channels to be incorporated into computational models of neurons.
ORCID iDs
Williams, Stephen R. and Wozny, Christian ORCID: https://orcid.org/0000-0003-4220-2033;-
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Item type: Article ID code: 60705 Dates: DateEvent15 March 2011Published2 February 2011AcceptedSubjects: Science > Natural history > Biology Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 17 May 2017 11:46 Last modified: 11 Nov 2024 11:18 URI: https://strathprints.strath.ac.uk/id/eprint/60705