Picture of boy being examining by doctor at a tuberculosis sanatorium

Understanding our future through Open Access research about our past...

Strathprints makes available scholarly Open Access content by researchers in the Centre for the Social History of Health & Healthcare (CSHHH), based within the School of Humanities, and considered Scotland's leading centre for the history of health and medicine.

Research at CSHHH explores the modern world since 1800 in locations as diverse as the UK, Asia, Africa, North America, and Europe. Areas of specialism include contraception and sexuality; family health and medical services; occupational health and medicine; disability; the history of psychiatry; conflict and warfare; and, drugs, pharmaceuticals and intoxicants.

Explore the Open Access research of the Centre for the Social History of Health and Healthcare. Or explore all of Strathclyde's Open Access research...

Image: Heart of England NHS Foundation Trust. Wellcome Collection - CC-BY.

454 pyrosequencing assessment of biodegradative bacteria from thermal hydrolysis processes

Colosimo, Fabrizio and Lord, Richard and Thomas, Russell and Lloyd, Jonathan R. and Boothman, Christopher and Kalin, Robert M (2017) 454 pyrosequencing assessment of biodegradative bacteria from thermal hydrolysis processes. Energy and Environment Research. ISSN 1927-0577 (In Press)

Text (Colosimo-etal-EER-2017-454-pyrosequencing-assessment-of-biodegradative-bacteria-from-thermal-hydrolysis-processes)
Accepted Author Manuscript
License: Creative Commons Attribution 3.0 logo

Download (572kB) | Preview


Anaerobic treatment process is a cost-effective method for treating organic wastes, since the biogas formed can be used for heat/electricity production and the digester residues can be recycled for other applications. An innovative use of the digestate could be as biodegradative and methanogenic inoculum for the stimulation of methane production in gas-producing or depleted wells. The microbial communities involved in the biodegradation of petrochemical waste are similar to the indigenous microorganisms typically found in unconventional basins. These communities also follow the same cascade of reactions: from the initial breakdown of complex molecules to the production of intermediate compounds used by methanogens. This study carried out a culture-independent assessment of the bacterial community composition of a digestate from the Bran Sands Advanced Digestion Facility (Middleborough, UK) and compared the results with the microbial populations found in unconventional gas basins. The 454 pyrosequencing analyses revealed a bacterial community dominated by Thermotogae, Bacteroidia, Clostridia and Synergistia, which are typically found in unconventional gas systems. The classification of nucleotide sequence reads and assembled contigs revealed a genetic profile characteristic for an anaerobic microbial consortium running fermentative metabolic pathways. The assignment of numerous sequences was related to hydrocarbon decomposition and digestion of cellulosic material, which indicates that the bacterial community is engaged in hydrolysis of plant-derived material. The bacterial community composition suggest that the effluent of the digester can be used as a biodegradative inoculum for the stimulation of methane generation in unconventional wells, where events of microbial methanogenesis have been previously observed.