Improved negative selection protocol for Plasmodium berghei in the rodent malarial model

Orr, Rachael Y and Philip, Nisha and Waters, Andrew P (2012) Improved negative selection protocol for Plasmodium berghei in the rodent malarial model. Malaria Journal, 11. 103. ISSN 1475-2875 (https://doi.org/10.1186/1475-2875-11-103)

[thumbnail of Orr-etal-MJ-2012-Improved-negative-selection-protocol-for-Plasmodium-berghei-in-the-rodent-malarial-model]
Preview
 Text. Filename: Orr_etal_MJ_2012_Improved_negative_selection_protocol_for_Plasmodium_berghei_in_the_rodent_malarial_model.pdf
Accepted Author Manuscript
License: Creative Commons Attribution 2.5 logo
Download (1MB)| Preview

Abstract

An improved methodology is presented here for transgenic Plasmodium berghei lines that express the negative selectable marker yFCU (a bifunctional protein that combines yeast cytosine deaminase and uridyl phosphoribosyl transferase (UPRT)) and substitutes delivery of selection drug 5-fluorocytosine (5FC) by intraperitoneal injection for administration via the drinking water of the mice. The improved methodology is shown to be as effective, less labour-intensive, reduces animal handling and animal numbers required for successful selection thereby contributing to two of the "three Rs" of animal experimentation, namely refinement and reduction.

  • Item type:Article
    ID code:60637
    Dates:
    Date
    Event
    31 March 2012
    Published
    Notes:Rachael Y Cameron Nee Rachael Y Orr
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:08 May 2017 10:30
    Last modified:11 Nov 2024 11:09
    URI:https://strathprints.strath.ac.uk/id/eprint/60637
CORE (COnnecting REpositories)