A tandem enzymatic sp2-C-methylation process : coupling in situ S-adenosyl-L-methionine formation with methyl transfer

Sadler, Joanna C and Humphreys, Luke D and Burley, Glenn and Snajdrova, Radka (2017) A tandem enzymatic sp2-C-methylation process : coupling in situ S-adenosyl-L-methionine formation with methyl transfer. Chembiochem. ISSN 1439-4227 (https://doi.org/10.1002/cbic.201700115)

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Abstract

A one-pot, two-step biocatalytic platform for the regiospecfic C-methylation and C-ethylation of aromatic substrates is described. The tandem process utilizes SalL (Salinospora tropica) for in situ synthesis of S-adenosyl-L-methionine (SAM), followed by alkylation of aromatic substrates using the C-methyltransferase NovO (Streptomyces spheroides). Application of this methodology is demonstrated by the regiospecific labelling of aromatic substrates via the transfer of methyl, ethyl and isotopically-labelled 13CH3, 13CD3 and CD3 groups from their corresponding SAM analogues formed in situ.