A tandem enzymatic sp2-C-methylation process : coupling in situ S-adenosyl-L-methionine formation with methyl transfer

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Sadler, Joanna C and Humphreys, Luke D and Burley, Glenn and Snajdrova, Radka (2017) A tandem enzymatic sp2-C-methylation process : coupling in situ S-adenosyl-L-methionine formation with methyl transfer. Chembiochem. ISSN 1439-4227 (https://doi.org/10.1002/cbic.201700115)

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Abstract

A one-pot, two-step biocatalytic platform for the regiospecfic C-methylation and C-ethylation of aromatic substrates is described. The tandem process utilizes SalL (Salinospora tropica) for in situ synthesis of S-adenosyl-L-methionine (SAM), followed by alkylation of aromatic substrates using the C-methyltransferase NovO (Streptomyces spheroides). Application of this methodology is demonstrated by the regiospecific labelling of aromatic substrates via the transfer of methyl, ethyl and isotopically-labelled 13CH3, 13CD3 and CD3 groups from their corresponding SAM analogues formed in situ.

ORCID iDs

Sadler, Joanna C ORCID logoORCID: https://orcid.org/0000-0002-8442-0760, Humphreys, Luke D, Burley, Glenn ORCID logoORCID: https://orcid.org/0000-0002-4896-113X and Snajdrova, Radka;
  • Item type:Article
    ID code:60384
    Dates:
    Date
    Event
    31 March 2017
    Published
    31 March 2017
    Published Online
    31 March 2017
    Accepted
    Notes:This is the peer reviewed version of the following article: Sadler, J. C., Humphreys, L. D., Burley, G., & Snajdrova, R. (2017). A tandem enzymatic sp2-C-methylation process: coupling in situ S-adenosyl-L-methionine formation with methyl transfer. Chembiochem, which has been published in final form at https://doi.org/10.1002/cbic.201700115. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Technology and Innovation Centre > Bionanotechnology
    Depositing user: Pure Administrator
    Date deposited:03 Apr 2017 11:04
    Last modified:02 Feb 2025 12:23
    URI:https://strathprints.strath.ac.uk/id/eprint/60384
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