Automated protein biomarker analysis : on-line extraction of clinical samples by molecularly imprinted polymers

Rossetti, Cecilia and Switnicka-Plak, Magdalena and Grønhaug Halvorsen, Trine and Cormack, Peter A.G. and Sellergren, Börje and Reubsaet, Léon (2017) Automated protein biomarker analysis : on-line extraction of clinical samples by molecularly imprinted polymers. Scientific Reports, 7. 44298. ISSN 2045-2322

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    Abstract

    Robust biomarker quantification is essential for the accurate diagnosis of diseases and is of great value in cancer management. In this paper, an innovative diagnostic platform is presented which provides automated molecularly imprinted solid-phase extraction (MISPE) followed by liquid chromatography-mass spectrometry (LC-MS) for biomarker determination using ProGastrin Releasing Peptide (ProGRP), a highly sensitive biomarker for Small Cell Lung Cancer, as a model. Molecularly imprinted polymer microspheres were synthesized by precipitation polymerization and analytical optimization of the most promising material led to the development of an automated quantification method for ProGRP. The method enabled analysis of patient serum samples with elevated ProGRP levels. Particularly low sample volumes were permitted using the automated extraction within a method which was time-efficient, thereby demonstrating the potential of such a strategy in a clinical setting.