2D-IR spectroscopy shows that optimised DNA minor groove binding of Hoechst33258 follows an induced fit model

Ramakers, Lennart A. I. and Hithell, Gordon and May, John J. and Greetham, Gregory M. and Donaldson, Paul M. and Towrie, Michael and Parker, Anthony W. and Burley, Glenn and Hunt, Neil (2017) 2D-IR spectroscopy shows that optimised DNA minor groove binding of Hoechst33258 follows an induced fit model. Journal of Physical Chemistry B. ISSN 1520-6106 (https://doi.org/10.1021/acs.jpcb.7b00345)

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The induced fit binding model describes a conformational change occurring when a small molecule binds to its biomacromolecular target. The result is enhanced non-covalent interactions between ligand and biomolecule. Induced fit is well-established for small molecule-protein interactions, but its relevance to small molecule-DNA binding is less clear. We investigate the molecular determinants of Hoechst33258 binding to its preferred A-tract sequence relative to a sub-optimal alternating A-T sequence. Results from 2-dimensional infrared spectroscopy, which is sensitive to H-bonding and molecular structure changes, show that Hoechst33258 binding results in loss of minor groove spine of hydration in both sequences, but an additional perturbation of the base propeller twists occurs in the A-tract binding region. This induced fit maximizes favourable ligand-DNA enthalpic contributions in the optimal binding case and demonstrates that controlling the molecular details that induce subtle changes in DNA structure may hold the key to designing next-generation DNA-binding molecules.


Ramakers, Lennart A. I. ORCID logoORCID: https://orcid.org/0000-0002-1844-9936, Hithell, Gordon ORCID logoORCID: https://orcid.org/0000-0001-9107-3229, May, John J. ORCID logoORCID: https://orcid.org/0000-0001-7796-2134, Greetham, Gregory M., Donaldson, Paul M., Towrie, Michael, Parker, Anthony W., Burley, Glenn and Hunt, Neil ORCID logoORCID: https://orcid.org/0000-0001-7400-5152;