The Shigella ProU system is required for osmotic tolerance and virulence

Mahmoud, Rasha Y. and Li, Wenqin and Eldomany, Ramadan A. and Emara, Mohamed and Yu, Jun (2017) The Shigella ProU system is required for osmotic tolerance and virulence. Virulence, 8 (4). pp. 362-374. 2016VIRULENCE0129R. ISSN 2150-5608 (https://doi.org/10.1080/21505594.2016.1227906)

[thumbnail of Mahmoud-etal-Virulence2016-Shigella-ProU-system-is-required-for-osmotic-tolerance-and-virulence]
Preview
 Text. Filename: Mahmoud_etal_Virulence2016_Shigella_ProU_system_is_required_for_osmotic_tolerance_and_virulence.pdf
Accepted Author Manuscript
Download (852kB)| Preview

Abstract

To cope with hyperosmotic stress encountered in the environments and in the host, the pathogenic as well as non-pathogenic microbes use diverse transport systems to obtain osmoprotectants. To study the role of Shigella sonnei ProU system in response to hyperosmotic stress and virulence, we constructed deletion and complementation strains of proV and used an RNAi approach to silence the whole ProU operon. We compared the response between wild type and the mutants to the hyperosmotic pressure in vitro, and assessed virulence properties of the mutants using gentamicin protection assay as well as Galleria mellonella moth larvae model. In response to osmotic stress by either NaCl or KCl, S. sonnei highly up-regulates transcription of proVWX genes. Supplementation of betaine greatly elevates the growth of the wild type S. sonnei but not the proV mutants in M9 medium containing 0.2 M NaCl or 0.2 M KCl. The proV mutants are also defective in intracellular growth compared with the wild type. The moth larvae model of G. mellonella shows that either deletion of proV gene or knockdown of proVWX transcripts by RNAi significantly attenuates virulence. ProU system in S. sonnei is required to cope with osmotic stress for survival and multiplication in vitro and ex vivo, and for infection.

ORCID iDs

Mahmoud, Rasha Y., Li, Wenqin ORCID logoORCID: https://orcid.org/0000-0003-0953-3922, Eldomany, Ramadan A., Emara, Mohamed and Yu, Jun ORCID logoORCID: https://orcid.org/0000-0002-3673-6760;
  • Item type:Article
    ID code:57462
    Dates:
    Date
    Event
    19 May 2017
    Published
    22 September 2016
    Published Online
    18 August 2016
    Accepted
    26 April 2016
    Submitted
    Notes:The Version of Record of this manuscript has been published and is available in Virulence 2016 http://www.tandfonline.com/toc/kvir20/current © 2017 Taylor & Francis Mahmoud, R. Y., Li, W., Eldomany, R. A., Emara, M., & Yu, J. (2016). The Shigella ProU system is required for osmotic tolerance and virulence. Virulence, 8(4), 362–374. https://doi.org/10.1080/21505594.2016.1227906
    Subjects:Science > Microbiology
    Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
    Depositing user: Pure Administrator
    Date deposited:18 Aug 2016 12:00
    Last modified:17 Nov 2024 01:11
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/57462
CORE (COnnecting REpositories)