Comment on 'A novel method for fast and robust estimation of fluorescence decay dynamics using constrained least-square deconvolution with Laguerre expansion'

Zhang, Yongliang and Li, David Day-Uei (2017) Comment on 'A novel method for fast and robust estimation of fluorescence decay dynamics using constrained least-square deconvolution with Laguerre expansion'. Physics in Medicine and Biology, 62 (4). pp. 1632-1636. ISSN 0031-9155

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    Abstract

    This comment is to clarify that Poisson noise instead of Gaussian noise shall be included to assess the performances of least-squares deconvolution with Laguerre expansion (LSD-LE) for analysing fluorescence lifetime imaging (FLIM) data obtained from time-resolved systems. Moreover, we also corrected an equation in the paper. As the LSD-LE approach is rapid and has potential to be widely applied not only for diagnostic but for wider bioimaging applications, it is desirable to have precise noise models and equations.