Comment on 'A novel method for fast and robust estimation of fluorescence decay dynamics using constrained least-square deconvolution with Laguerre expansion'

Zhang, Yongliang and Li, David Day-Uei (2017) Comment on 'A novel method for fast and robust estimation of fluorescence decay dynamics using constrained least-square deconvolution with Laguerre expansion'. Physics in Medicine and Biology, 62 (4). pp. 1632-1636. ISSN 0031-9155 (https://doi.org/10.1088/1361-6560/aa522f)

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Abstract

This comment is to clarify that Poisson noise instead of Gaussian noise shall be included to assess the performances of least-squares deconvolution with Laguerre expansion (LSD-LE) for analysing fluorescence lifetime imaging (FLIM) data obtained from time-resolved systems. Moreover, we also corrected an equation in the paper. As the LSD-LE approach is rapid and has potential to be widely applied not only for diagnostic but for wider bioimaging applications, it is desirable to have precise noise models and equations.

ORCID iDs

Zhang, Yongliang and Li, David Day-Uei ORCID logoORCID: https://orcid.org/0000-0002-6401-4263;
CORE (COnnecting REpositories)