Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane

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Amor, Rumelo and Mahajan, Sumeet and Amos, William B. and McConnell, Gail; (2015) Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. SPIE, USA. ISBN 9781628414202 (https://doi.org/10.1117/12.2075644)

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Abstract

We demonstrate fluorescence excitation at multiple planes in a laser-scanning microscope by using the standing wave from a mirror placed close to the specimen. We have observed precise modulation of the standing waves close to a mirror, with a frequency proportional to the Stokes shift, corresponding to a moiré pattern between the excitation and emission standing-wave fields. We use standing-wave excitation to plot the exact contour maps of the red blood cell membrane, with an axial resolution of ≈90 nm. The method may prove useful in the study of diseases which involve the surface membrane of red blood cells.

ORCID iDs

Amor, Rumelo, Mahajan, Sumeet, Amos, William B. and McConnell, Gail ORCID logoORCID: https://orcid.org/0000-0002-7213-0686;
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