Fluorescence lifetime estimation method for incomplete decay

Yu, Hongqi and Li, David Day-Uei (2015) Fluorescence lifetime estimation method for incomplete decay. Electronics Letters. ISSN 0013-5194

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    Abstract

    A new incomplete decay signal model is proposed to describe the incomplete decay effects in a time-correlated single-photon counting (TCSPC) based fluorescence lifetime imaging (FLIM) system. Based on this model, we modified a MUltiple SIgnal Classification (MUSIC) algorithm to eliminate the incomplete decay effects. Monte Carlo simulations were carried out to demonstrate the performances of the proposed approach. Simulations show that the proposed method is insensitive to the laser pulse rate and has a larger lifetime dynamic range compared with previously reported approaches. As far as we know, this new method is the first non-fitting method that can resolve incomplete decay effects for multi-exponential decays.

    Author(s):Yu, Hongqi and Li, David Day-Uei ORCID logoORCID: https://orcid.org/0000-0002-6401-4263
    Item type:Article
    ID code:54376
    Keywords:decay signal model, decay effects, fluorescence lifetime imaging, Pharmacy and materia medica, Pharmacology, Toxicology and Pharmaceutics(all)
    Subjects:Medicine > Pharmacy and materia medica
    Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
    Depositing user: Pure Administrator
    Date deposited:30 Sep 2015 09:22
    Last modified:22 Aug 2019 01:37
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/54376
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