PclA, a pneumococcal collagen-like protein with selected strain distribution, contributes to adherence and invasion of host cells

Tools

Paterson, Gavin K. and Nieminen, Leena and Jefferies, Johanna M.C. and Mitchell, Tim J. (2008) PclA, a pneumococcal collagen-like protein with selected strain distribution, contributes to adherence and invasion of host cells. FEMS Microbiology Letters, 285 (2). pp. 170-176. ISSN 0378-1097 (https://doi.org/10.1111/j.1574-6968.2008.01217.x)

Full text not available in this repository.Request a copy

Abstract

Analysis of Streptococcus pneumoniae sequenced genomes revealed a region present only in selected strains consisting of two ORFs: a putative cell wall anchored protein and a putative transcriptional regulator. The cell wall anchored protein contains large regions of collagen-like repeats, the number of which varies between strains. We have therefore named this protein PclA for pneumococcal collagen-like protein A. The second gene, spr1404, encodes a putative transcriptional regulator. We examined the strain distribution of these two genes among a collection of clinical isolates from invasive pneumococcal disease and found them to be present in 39% of the strains examined. Strains were either positive for both genes or lacked both, with the two genes always present together in the same location of the genome. RT-PCR analysis revealed that pclA is transcribed in vitro, even in the absence of spr1404. Single deletion mutants lacking either gene were not attenuated in a mouse model of invasive pneumonia. However, the pclA mutant was defective in adherence and invasion of host cells in vitro.

ORCID iDs

Paterson, Gavin K., Nieminen, Leena ORCID logoORCID: https://orcid.org/0000-0002-7172-7923, Jefferies, Johanna M.C. and Mitchell, Tim J.;
CORE (COnnecting REpositories)