Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches

Li, David and Chen, Yu (2015) Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. SPIE, Bellingham, WA. ISBN 9781628417012

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    Abstract

    A newly developed hardware-friendly non-iterative fluorescence lifetime imaging (FLIM) analysis method was verified in an FPGA chip. Its performances were also demonstrated on two-photon FLIM images of gold nanorods (GNRs)-Cy5 labelled Hela cells. The results obtained by the proposed method can be presented in a polor plot to be compared to the widely used phasor (Phasor) approach. Combining our method with Phasor will be very useful in FLIM analysis.